10843555001

Hygromycin B

from Streptomyces hygroscopicus

• Synonim(y): Hygromycin B from Streptomyces hygroscopicus
• Wzór empiryczny (zapis Hilla): C₂₀H₃₇N₃O₁₃
• Numer CAS: 31282-04-9
• Masa cząsteczkowa: 527.52
• Numer MDL: MFCD06795479
• Kod UNSPSC: 12352200
• Identyfikator substancji w PubChem: 329749191

Właściwości

• pochodzenie biologiczne: Streptomyces hygroscopicus
• sterylność: non-sterile; 0.2 μm filtered
• Próba: 80% (HPLC)
• Postać: buffered aqueous solution
• skład: Hygromycin B, >80% HPLC
• opakowanie: pkg of 20 mL (1 g)
• producent / nazwa handlowa: Roche
• stężenie: 50 mg/mL
• metody: transfection: suitable
• ciąg SMILES: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
• Klucz InChI: GRRNUXAQVGOGFE-XKIAHZFYSA-N

Opis

Opis ogólny

• Formula: C₂₀H₃₇N₃O₁₃
• LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
• Molecular weight: Mr = 527.5
• Working concentration: 50 - 1,000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.

Zastosowanie

Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain the phenotype of eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).

Działania biochem./fizjol.

Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Jakość

Purity: >80% (HPLC)

Definicja jednostki

Unit Assay: Unit/mg
We do not have this information.

Postać fizyczna

Solution, 50 mg/ml in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane

Uwaga dotycząca przygotowania

Working concentration: Recommended concentration for the selection of resistant cells is 50 to 1000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be determined experimentally, and may vary with the type of cell used.

Przechowywanie i stabilność

Store at 2 to 8 °C. (solution)

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

Solution, 50 mg/ml, in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane.

This page may contain text that has been machine translated.

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS05,GHS06,GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H301,H312,H317,H318,H330,H334
• Zwroty wskazujące środki ostrożności: P260 - P280 - P301 + P310 + P330 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P342 + P311 - P403 + P233
• Klasyfikacja zagrożeń: Acute Tox. 1 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1
• Kod klasy składowania: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash