N6386

Neomycin trisulfate salt hydrate

powder, suitable for cell culture, BioReagent

• Synonim(y): Antibiotic 10676, Neomycin B
• Wzór empiryczny (zapis Hilla): C₂₃H₄₆N₆O₁₃ · 3H₂SO₄ · xH₂O
• Numer CAS: 1405-10-3
• Masa cząsteczkowa: 908.88 (anhydrous basis)
• Numer WE: 215-773-1
• Kod UNSPSC: 12352207
• Identyfikator substancji w PubChem: 24897673
• NACRES: NA.76

Właściwości

• product name: Neomycin trisulfate salt hydrate, powder, BioReagent, suitable for cell culture
• pochodzenie biologiczne: Streptomyces sp.
• linia produktu: BioReagent
• Postać: powder
• siła działania: ≥600 μg neomycin per mg (Dried basis)
• opakowanie: pkg of 100 g; pkg of 25 g; pkg of 5 g
• metody: cell culture | mammalian: suitable
• kolor: white to yellow
• rozpuszczalność: H₂O: 50 mg/mL
• spektrum działania antybiotyku: Gram-negative bacteria; Gram-positive bacteria
• Tryb działania: protein synthesis | interferes
• temp. przechowywania: 2-8°C
• ciąg SMILES: O.OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.NC[C@@H]1O[C@H](O[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)O[C@@H]3[C@@H](O)[C@H](N)C[C@H](N)[C@H]3O[C@H]4O[C@H](CN)[C@@H](O)[C@H](O)[C@H]4N)C(N)[C@@H](O)[C@@H]1O
• InChI: 1S/C23H46N6O13.3H2O4S.H2O/c24-2-7-13(32)15(34)10(28)21(37-7)40-18-6(27)1-5(26)12(31)20(18)42-23-17(36)19(9(4-30)39-23)41-22-11(29)16(35)14(33)8(3-25)38-22;3*1-5(2,3)4;/h5-23,30-36H,1-4,24-29H2;3*(H2,1,2,3,4);1H2/t5-,6+,7-,8+,9-,10-,11?,12+,13-,14-,15-,16-,17-,18-,19-,20-,21-,22-,23+;;;;/m1..../s1
• Klucz InChI: WHAGUNPVKDUVFV-QGTTWHFQSA-N

Opis

Opis ogólny

As a stock solution. Stock solutions should be filter sterilized and stored at 2-8°C. Stable at 37°C for 5 days.

Chemical structure: aminoglycoside

Zastosowanie

Neomycin trisulfate salt hydrate has been used:

• to determine Netrin1- encoding gene (Ntn1) protective effects against aminoglycoside antibiotics in cochlear explants
• for Escherichia coli antibiotic susceptibility test
• in bone marrow transplantation of mice

Działania biochem./fizjol.

Neomycin Trisulfateis an aminoglycoside antibiotic, which is produced by Streptomyces containing a minimum of 85% neomycin B.It is used to study the cytotoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts and extraction of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins. Neomycin trisulfate functions as a selection agent for prokaryotic cells transformed using the neo selectable marker gene. This product is recommended for use in cell culture applications at 5 mL/L.

Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca²⁺ channels without affecting the Na⁺/Ca²⁺ antiporter in neurons.

Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria.

Przestroga

Stock solutions should be filter sterilized and stored at 2-8°C, and are stable at 37°C for 5 days. A 1 mg/mL neomycin solution in .1 M phosphate buffer, pH 8.0 should be used within 14 days when stored at 0-5°C. Solutions should be protected from light or moisture.

Uwaga dotycząca przygotowania

Neomycin sulfate is soluble in H₂O at 50 mg/mL, yielding a clear solution.

Inne uwagi

Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H317,H334
• Zwroty wskazujące środki ostrożności: P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312
• Klasyfikacja zagrożeń: Resp. Sens. 1 - Skin Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Faceshields, Gloves