MBD0015

DAPI ready made solution

For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL

• Synonim(y): 4′,6-Diamidino-2-phenylindole dihydrochloride, DAPI Dye, DAPI Stain, DNA Content Counterstain, 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride
• Wzór empiryczny (zapis Hilla): C₁₆H₁₅N₅ · 2HCl
• Numer CAS: 28718-90-3
• Masa cząsteczkowa: 350.25
• Beilstein: 4894417
• Numer MDL: MFCD00012681
• Kod UNSPSC: 41105500
• NACRES: NA.55

Właściwości

• Poziom jakości: 200
• Postać: liquid
• warunki przechowywania: protect from light
• stężenie: 1 mg/mL
• metody: transfection: suitable
• rozpuszczalność: water: soluble
• fluorescencja: λ_ex 340 nm; λ_em 488 nm (nur DAPI); λ_ex 364 nm; λ_em 454 nm (DAPI-DNA-Komplex)
• przydatność: suitable for nucleic acid staining
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• ciąg SMILES: Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
• InChI: 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
• Klucz InChI: FPNZBYLXNYPRLR-UHFFFAOYSA-N

Opis

Opis ogólny

DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.

Zastosowanie

DAPI Readymade Solution is provided at 1mg/mL concentration. It is recommended to dilute DAPI readymade solution 1:1000 with a desired buffer for a 1μg/mL working concentration (typical working concentration used in most applications is 0.1μg/mL-10μg/mL). Store DAPI solution (1mg/mL) -20°C protected from light. It is recommended to aliquot DAPI readymade solution and to avoid freeze thaw cycles.

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS).

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Działania biochem./fizjol.

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Cechy i korzyści

• Ready-to-use liquid, cell permeable dye.
• Can be used with live cells and fixed cells.
• Effective for fixed-cell staining and quantitation of DNA content.
• Optimal Counterstain when using fluorescent antibodies during fluorescence microscopy or high-content screening (HCS) applications.

Postać fizyczna

DAPI readymade solution is provided at 1mg/mL concentration in water

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H317
• Zwroty wskazujące środki ostrożności: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Klasyfikacja zagrożeń: Skin Sens. 1
• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable