D8417

4′,6-Diamidino-2-phenylindole dihydrochloride

powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC), suitable for fluorescence

• Synonim(y): 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride
• Wzór empiryczny (zapis Hilla): C₁₆H₁₅N₅ · 2HCl
• Numer CAS: 28718-90-3
• Masa cząsteczkowa: 350.25
• Beilstein: 4894417
• Numer WE: 249-186-7
• Numer MDL: MFCD00012681
• Kod UNSPSC: 41116130
• Identyfikator substancji w PubChem: 24894213
• NACRES: NA.75

Właściwości

• linia produktu: BioReagent
• Poziom jakości: 200
• Próba: ≥98% (HPLC and TLC)
• Postać: powder
• metody: cell culture | mammalian: suitable
• rozpuszczalność: H₂O: 20 mg/mL; PBS: insoluble
• ε (współczynnik ekstynkcji): 30 at 263 nm in H₂O
• fluorescencja: λ_ex 340 nm; λ_em 488 nm (nur DAPI); λ_ex 364 nm; λ_em 454 nm (DAPI-DNA-Komplex)
• przydatność: suitable for fluorescence
• temp. przechowywania: 2-8°C
• ciąg SMILES: Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
• InChI: 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
• Klucz InChI: FPNZBYLXNYPRLR-UHFFFAOYSA-N

Opis

Opis ogólny

4′,6-Diamidino-2-phenylindole dihydrochloride is a fluorescent dye for staining DNA. It strongly interacts with the dAT-rich (deoxy adenine-thymine) regions of the DNA.

Heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.

Zastosowanie

4′,6-Diamidino-2-phenylindole dihydrochloride has been used in staining of various cells in immunocytochemistry and cytochemistry, human subcutaneous adipose tissues for fluorescence imaging, embryo sections for immunofluorescence.

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Działania biochem./fizjol.

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Przestroga

Protect from light.

Inne uwagi

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H315,H317,H335
• Zwroty wskazujące środki ostrożności: P261 - P264 - P271 - P272 - P280 - P302 + P352
• Klasyfikacja zagrożeń: Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
• Organy docelowe: Respiratory system
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)