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MBD0001

Sigma-Aldrich

Microbial DNA standard from Akkermansia muciniphila

Suitable for PCR, sequencing and NGS, 10 ng/μL

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About This Item

Kod UNSPSC:
41105500
NACRES:
NA.24

Poziom jakości

200

Postać

liquid

stężenie

10 ng/μL

metody

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

Warunki transportu

ambient

temp. przechowywania

−20°C

Powiązane kategorie

Opis ogólny

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses.1 Akkermansia muciniphila genomic DNA microbial standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.
Akkermansia muciniphila is a gram negative, anaerobic, non motile, non spore forming and oval shaped bacterium. A. muciniphila inhabits the gastrointestinal tracts of more than 90% of adults and constitutes 1 to 4% of the fecal microbiota.2 A. muciniphila level was suggested to be a biomarker for a healthy intestin. 3
A. muciniphila degrades mucus and utilizes it as a carbon/nitrogen source. Consequently, the host produces additional mucus while the bacterium produces oligosaccharides and Short Chain Fatty Acids (SCFAs) that can be utilized by the host and trigger the immune system. An additional protective effect of the SCFA is stimulation of mucus-associated microbiota growth, that serves as a barrier against penetration of pathogens to intestinal cells. 2,4

Read here how to use our standards to ensure data integrity for your microbiome research.

Zastosowanie

Suitable for Quantitative standard for PCR, Sequencing and NGS

Cechy i korzyści

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Postać fizyczna

Liquid - The genomic DNA is provided at ≥10 ng/μL concentration in TE buffer pH 8.0

Inne uwagi

It is recommended to avoid freeze thaw cycles of this product.
This page may contain text that has been machine translated.

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Clara Belzer et al.
The ISME journal, 6(8), 1449-1458 (2012-03-23)
The human intestinal tract is colonized by a myriad of microbes that have developed intimate interactions with the host. In healthy individuals, this complex ecosystem remains stable and resilient to stressors. There is significant attention on the understanding of the
Chin Wen Png et al.
The American journal of gastroenterology, 105(11), 2420-2428 (2010-07-22)
Mucosa-associated bacteria are increased in inflammatory bowel disease (IBD), which suggests the possibility of an increased source of digestible endogenous mucus substrate. We hypothesized that mucolytic bacteria are increased in IBD, providing increased substrate to sustain nonmucolytic mucosa-associated bacteria. Mucolytic
Muriel Derrien et al.
International journal of systematic and evolutionary microbiology, 54(Pt 5), 1469-1476 (2004-09-25)
The diversity of mucin-degrading bacteria in the human intestine was investigated by combining culture and 16S rRNA-dependent approaches. A dominant bacterium, strain MucT, was isolated by dilution to extinction of faeces in anaerobic medium containing gastric mucin as the sole
J Paul Brooks et al.
BMC microbiology, 15, 66-66 (2015-04-17)
Characterizing microbial communities via next-generation sequencing is subject to a number of pitfalls involving sample processing. The observed community composition can be a severe distortion of the quantities of bacteria actually present in the microbiome, hampering analysis and threatening the

Produkty

Human Microbiome Research Workflow: An Overview of the Role in Human Health and Disease

An overview of human microbiome research, workflow challenges, sequencing, library production, data analysis, and available microbiome reagents to support your research.

DNA Standards for Metagenomic Research into Microbiome Communities

The use of standards is critical to the integrity of metagenomics research. Learn how DNA standards for bacteria, fungi, and viruses are applied to studying the microbiome. Choose standards for E. coli and other key species, as well as mixed community standards.

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