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Merck

M7292

Sigma-Aldrich

M16 Medium

With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered

Synonim(y):

Cell Culture Medium, Cell Culture Medium M16, M16 Medium Solution

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About This Item

Kod UNSPSC:
12352207
NACRES:
NA.75

sterylność

sterile-filtered

Postać

liquid

metody

cell culture | embryo: suitable

zanieczyszczenia

endotoxin, tested

komponenty

NaHCO3: 2.101 g/L
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)

Warunki transportu

ambient

temp. przechowywania

2-8°C

Zastosowanie

M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

Jakość

Presence of particulates in solution will not affect product′s performance.

Rekonstytucja

Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.

Komentarz do analizy

This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Protokoły

Creating Transgenic Mice using CRISPR-Cas9 Genome Editing

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