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M7167
M2 medium
With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture
Synonim(y):
Cell Culture M2, Cell Culture Medium, M2 Medium Solution
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About This Item
Polecane produkty
Poziom jakości
sterylność
sterile-filtered
Formularz
liquid
warunki przechowywania
protect from light
metody
cell culture | embryo: suitable
zanieczyszczenia
endotoxin, tested
komponenty
HEPES: 5.42726 g/L
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
Warunki transportu
ambient
temp. przechowywania
2-8°C
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Zastosowanie
Recommended for manipulation of mouse embryos at ambient temperature. M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.
M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.
M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.
Jakość
Presence of particulates in solution will not affect product′s performance.
Rekonstytucja
Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.
Komentarz do analizy
This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.
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polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Protokoły
Creating Transgenic Mice using CRISPR-Cas9 Genome Editing
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