About This Item
Polecane produkty
sterylność
non-sterile
Formularz
light oil
warunki przechowywania
protect from light
współczynnik refrakcji
n20/D 1.467 (lit.)
gęstość
0.84 g/mL at 25 °C (lit.)
Format
neat
Klucz InChI
AEOVEGJBKQQFOP-DDVLFWKVSA-L
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Zastosowanie
Mineral oil has been used as an overlay in microdrop embryo cultures.
Przestroga
This product is not sterile. Mineral oil is difficult to sterilize. It can only be sterilized by careful filtration of the warmed oil. It is assumed antibiotics will be added to the cell culture medium. Otherwise, Prod. No. M5310 should be used, as it is sterile filtered.
Komentarz do analizy
This material is tested as an overlay in microdrop embryo cultures. (All embryo tested materials are tested using mouse embryos.) Typically BCF1 x BDF1 hybrids are used. The oil is primarily used to prevent evaporation of the medium, particiularly when performing embryo manipulations.1 The procedure is as follows: 1-cell (BCF1 X BDF1) embryos were cultured in triplicate microdrops of embryo-tested HTF (Human Tubal Fluid) medium supplemented with 0.4% BSA. For a valid assay, at least 70% of 1-cell stage control embryos must develop to the blastocyst stage within 96 hours. For product release, at least 80% of 1-cell stage test embryos must develop to the blastocyst stage within 96 hours with the tested material.
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produkt podobny
Numer produktu
Opis
Cennik
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
No data available
Temperatura zapłonu (°C)
No data available
Środki ochrony indywidualnej
Eyeshields, Gloves
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Protokoły
Summary application report for analysis of moisture in Mineral oil
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