L6011
Lipopolysaccharides from Salmonella enterica serotype enteritidis
purified by phenol extraction
Synonim(y):
LPS
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About This Item
Polecane produkty
pochodzenie biologiczne
Salmonella enterica (Serotype enteritidis)
Poziom jakości
Formularz
lyophilized powder
oczyszczone przez
phenol extraction
zanieczyszczenia
<3% Protein (Lowry)
kolor
white to faint yellow
rozpuszczalność
water: 0.90-1.10 mg/mL, slightly hazy to turbid, colorless to faintly yellow
Warunki transportu
ambient
temp. przechowywania
2-8°C
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Opis ogólny
This product is phenol extracted from Salmonella enteritidis. The source strain is ATCC 13076. Lipopolysaccharide from Salmonella enteritidis is mutagenic in murine spleen cells and causes a plaque-forming response in those cells.
Zastosowanie
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4).
Działania biochem./fizjol.
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Uwaga dotycząca przygotowania
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Inne uwagi
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
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produkt powiązany
Numer produktu
Opis
Cennik
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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