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Key Documents
H7541
IL-10 human
HumanKine®, recombinant, Xeno-free, >95% (SDS-PAGE), suitable for cell culture
Synonim(y):
IL-10
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About This Item
Polecane produkty
product name
Interleukin-10 human, IL-10, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
pochodzenie biologiczne
human
Poziom jakości
rekombinowane
expressed in HEK 293 cells
Próba
≥95% (SDS-PAGE)
Postać
lyophilized powder
siła działania
≤1.5 ng/mL EC50
jakość
endotoxin tested
masa cząsteczkowa
dimer 17 kDa (glycosylated)
metody
cell culture | mammalian: suitable
zanieczyszczenia
≤1EU/mg
numer dostępu UniProt
temp. przechowywania
−20°C
informacje o genach
human ... IL10(3586)
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Opis ogólny
Interleukin-10 gene with five exons is mapped to human chromosome 1. The gene codes for 35 kD protein containing 178 amino acids. IL-10 is a homodimer with two, non-covalently bonded monomers.
Zastosowanie
Interleukin-10 human has been used to study its effect on activation of endogenous signal transducer and activator of transcription 1 (STAT1)/STAT3 in the knock-in, in A549 human lung adenocarcinoma cell line.
Działania biochem./fizjol.
IL-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1+ (CD5+), and normal B cells. In human, the cellular sources of IL-10 consist of CD4+ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.
Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors.
Postać fizyczna
Lyophilized from a 0.2 μm filtered solution of 10mM Tris-HCl pH 7.4 + 150mM NaCl
Uwaga dotycząca przygotowania
HumanKine Interleukin-10 (IL-10) is expressed in human HEK 293 cells as a glycosylated, non-disulfide linked homodimer with an apparent molecular mass of 17 kDa due to glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
Komentarz do analizy
The specific activity was determined by the dose-dependent stimulation of the proliferation of MC/9 cells (mouse master cell line) in presence of 200 pg/mL IL-4.
Informacje prawne
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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