H7541
IL-10 human
HumanKine®, recombinant, Xeno-free, >95% (SDS-PAGE), suitable for cell culture
Synonim(y):
Interleukina-10 ludzka
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About This Item
Polecane produkty
Nazwa produktu
Interleukin-10 human, IL-10, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
pochodzenie biologiczne
human
Poziom jakości
rekombinowane
expressed in HEK 293 cells
Próba
≥95% (SDS-PAGE)
Formularz
lyophilized powder
siła działania
≤1.5 ng/mL EC50
jakość
endotoxin tested
masa cząsteczkowa
dimer 17 kDa (glycosylated)
metody
cell culture | mammalian: suitable
zanieczyszczenia
≤1EU/mg
numer dostępu UniProt
temp. przechowywania
−20°C
informacje o genach
human ... IL10(3586)
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Opis ogólny
Gen interleukiny-10 z pięcioma eksonami jest zmapowany na ludzkim chromosomie 1. Gen koduje białko o masie 35 kD zawierające 178 aminokwasów. IL-10 jest homodimerem z dwoma niekowalencyjnie związanymi monomerami.
Zastosowanie
Interleukina-10 została wykorzystana do zbadania jej wpływu na aktywację endogennego transduktora sygnału i aktywatora transkrypcji 1 (STAT1)/STAT3 w linii komórkowej A549 ludzkiego gruczolakoraka płuc.
Działania biochem./fizjol.
IL-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1+ (CD5+), and normal B cells. In human, the cellular sources of IL-10 consist of CD4+ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.
Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors.
Regulates lymphoid and myeloid cell functions; blocks the activation of cytokine synthesis and several accessory functions of macrophages; enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion.
Postać fizyczna
Liofilizowany z 0,2 μm przefiltrowanego roztworu 10 mM Tris-HCl pH 7,4 + 150 mM NaCl
Uwaga dotycząca przygotowania
HumanKine Interleukin-10 (IL-10) is expressed in human HEK 293 cells as a glycosylated, non-disulfide linked homodimer with an apparent molecular mass of 17 kDa due to glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
Komentarz do analizy
The specific activity was determined by the dose-dependent stimulation of the proliferation of MC/9 cells (mouse master cell line) in presence of 200 pg/mL IL-4.
Informacje prawne
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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