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Merck

G2904

Sigma-Aldrich

Monoclonal Anti-Goat/Sheep IgG antibody produced in mouse

clone GT-34, ascites fluid

Synonim(y):

Monoclonal Anti-Goat/Sheep IgG

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.46

pochodzenie biologiczne

mouse

białko sprzężone

unconjugated

forma przeciwciała

ascites fluid

rodzaj przeciwciała

secondary antibodies

klon

GT-34, monoclonal

zawiera

15 mM sodium azide

reaktywność gatunkowa

sheep, goat, bovine

spodziewany brak reakcji z

rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human

metody

indirect ELISA: 1:10,000-1:20,000

izotyp

IgG1

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

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Opis ogólny

IgG is the main type of antibody in human serum, secreted by the B cells. It is found in blood. IgG consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are linked by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.
The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.

Specyficzność

Monoclonal Anti-Goat/Sheep IgG recognizes an epitope located on the heavy chain of goat IgG1 and IgG2. The antibody shows strong cross-reactivity with sheep IgG and weak cross-reactivity with bovine IgG. No cross-reactivity is observed with IgG derived from human, guinea pig, rat, horse, dog, chicken, pig, mouse, rabbit, or cat.

Zastosowanie

Monoclonal Anti-Goat/Sheep IgG antibody produced in mouse has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunoprecipitation
  • immunoelectrophoresis

Działania biochem./fizjol.

IgG stimulates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a crucial role in antibody-dependent cell-mediated cytotoxicity (ADCC). IgG might be associated with allergy. It has the longest serum half life.

Postać fizyczna

The product is provided as ascites fluid with 0.1% sodium azide as preservative.

Przechowywanie i stabilność

For continuous use, store at 2–8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

nwg

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Tom N McNeilly et al.
Veterinary research, 44, 70-70 (2013-08-24)
Teladorsagia circumcincta is an important pathogenic nematode of sheep. It has been demonstrated previously that stimulation of murine T lymphocytes with excretory-secretory (ES) products derived from fourth stage larvae of T. circumcincta (Tci-L4-ES) results in de novo expression of Foxp3
Seasonal variations of phagocytic response, immunoglobulin G (IgG) and plasma cortisol levels in Dhofari goats
Al-Busaidi R, et al.
Small Ruminant Research, 79(2-3), 118-123 (2008)
Guillaume Sallé et al.
International journal for parasitology, 48(5), 395-402 (2018-03-15)
Some nematode species are economically important parasites of livestock, while others are important human pathogens causing some of the most important neglected tropical diseases. In both humans and animals, anthelmintic drug administration is the main control strategy, but the emergence
Brett Roberts et al.
Veterinary research, 44(1), 111-111 (2013-12-03)
With the problem of parasitic nematode drug resistance increasing, vaccine development offers an alternative sustainable control approach. For some parasitic nematodes, native extracts enriched for specific proteins are highly protective. However, recombinant forms of these proteins have failed to replicate
A revised nomenclature for allergy: an EAACI position statement from the EAACI nomenclature task force
Johansson SGO, et al.
Allergy, 56(9), 813-824 (2001)

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