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A9452
Anti-Goat/Sheep IgG−Peroxidase antibody, Mouse monoclonal
clone GT-34, purified from hybridoma cell culture
Synonim(y):
Monoclonal Anti-Goat/Sheep IgG
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About This Item
Kod UNSPSC:
12352203
NACRES:
NA.46
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
białko sprzężone
peroxidase conjugate
forma przeciwciała
purified from hybridoma cell culture
rodzaj przeciwciała
secondary antibodies
klon
GT-34, monoclonal
Formularz
lyophilized powder
reaktywność gatunkowa
sheep, goat, bovine
spodziewany brak reakcji z
rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human
opakowanie
vial of 0.5 mL
metody
direct ELISA: 1:30,000
dot blot: 1:160,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
izotyp
IgG1
temp. przechowywania
2-8°C
docelowa modyfikacja potranslacyjna
unmodified
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Opis ogólny
IgG is the most abundant antibody in human serum.It is found in blood and secreted by the B cells. It consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are connected by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.
IgGs are glycoprotein antibodies that modulate several immune responses. Goat or sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-goat/sheep IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Mouse monoclonal anti-goat/sheep IgG-peroxidase antibody shows strong cross-reactivity with sheep IgG and cross-reacts with bovine IgG. No cross-reaction of the unconjugated monoclonal antibody is observed with human serum and tissue components. Moreover, the antibody does not react with the following species: guinea pig, rat, mouse, rabbit, horse, dog, chicken, pig and cat.
Mouse monoclonal anti-goat/sheep IgG-peroxidase antibody shows strong cross-reactivity with sheep IgG and cross-reacts with bovine IgG. No cross-reaction of the unconjugated monoclonal antibody is observed with human serum and tissue components. Moreover, the antibody does not react with the following species: guinea pig, rat, mouse, rabbit, horse, dog, chicken, pig and cat.
The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.
Zastosowanie
ELISA using HRP-conjugated monoclonal anti-goat/sheep IgG as the secondary was performed on human T cells.
Mouse monoclonal anti-goat/sheep IgG-peroxidase antibody has been used for western blot (1:5,000) and ELISA. The product can also be used for dot blot (1:30,000) and IHC (1:100).
Działania biochem./fizjol.
IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC). IgG enables transplacental transport. It has the longest serum half life. It might be associated with allergy.
Postać fizyczna
Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT
Uwaga dotycząca przygotowania
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Skin Sens. 1
Kod klasy składowania
13 - Non Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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