GE17-5282-02

GSTrap^™ High Performance

Cytiva 17-5282-02, pack of 5 × 5 mL

• Kod UNSPSC: 23151817
• NACRES: NA.56

Właściwości

• ligand: glutathione
• opakowanie: pack of 5 × 5 mL
• producent / nazwa handlowa: Cytiva 17-5282-02
• Parametry: <20 mL/min flow rate; 42 psi (H₂O at room temperature.)
• wymiary złoża: 16 mm × 25 mm
• objętość złoża: 5 mL
• kolumna śr. wewn.: 16 mm
• macierz: highly cross-linked 6% agarose
• śr. rozm. cząst.: 34 μm
• cleaning: 3-12
• working range: 3-12
• pojemność: >7 mg/mL binding capacity (medium)

Opis

Opis ogólny

GSTrap^™ HP columns are prepacked with Glutathione Sepharose^™ High Performance medium and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.

GSTrap^™ HP 1-ml and 5-ml columns are prepacked with Glutathione Sepharose^™ High Performance medium, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose^™ High Performance. GST-tagged proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer.

GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Cechy i korzyści

• High resolution purification of GST-tagged proteins.
• Mild elution conditions preserving protein antigenicity and function.
• Fast, one-step purification.
• Easy to use with syringe, pump or a chromatography system.

Przechowywanie i stabilność

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Komentarz do analizy

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Inne uwagi

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informacje prawne

GSTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS02
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H226
• Zwroty wskazujące środki ostrożności: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Kod klasy składowania: 3 - Flammable liquids