GE17-0998-01
Heparin Sepharose™ 6 Fast Flow
Cytiva 17-0998-01, pack of 50 mL
Synonim(y):
Heparin Sepharose 6 Fast Flow
About This Item
Polecane produkty
ligand
porcine heparin
opakowanie
pack of 50 mL
producent / nazwa handlowa
Cytiva 17-0998-01
macierz
6% cross-linked agarose
wielkość cząstki
45-165 μm
średnia średnica
90 μm
cleaning in place
4-13
working range
4-12
przydatność
suitable for bioprocess medium
Powiązane kategorie
Opis ogólny
Heparin Sepharose™ 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.
Heparin Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand.
This medium allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins. Additional applications include DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors.
Heparin Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Heparin Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Cechy i korzyści
- Industrial purification of antithrombin III, coagulation factors and other plasma proteins
- Coupling chemistry gives chemically-stable ligand attachment. Stability of medium solely dictated by heparin ligand.
- BioProcess medium supported for industrial applications and well-established in approved processes
Przechowywanie i stabilność
Komentarz do analizy
Informacje prawne
Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Kod klasy składowania
3 - Flammable liquids
Certyfikaty analizy (CoA)
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Produkty
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from GE Healthcare.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protokoły
DNA binding proteins form an extremely diverse class of proteins sharing a single characteristic, their ability to bind to DNA. Functionally the group can be divided into those responsible for the replication and orientation of the DNA such as histones, nucleosomes and replicases and those involved in transcription such as RNA/DNA polymerases, transcriptional activators and repressors and restriction enzymes.
How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.
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