GE17-0406-01
HiTrap® Heparin High Performance
Cytiva 17-0406-01, pack of 5 × 1 mL
Synonim(y):
Kolumna powinowactwa do heparyny
About This Item
Polecane produkty
ligand
porcine heparin
opakowanie
pack of 5 × 1 mL
producent / nazwa handlowa
Cytiva 17-0406-01
Parametry
<4 mL/min flow rate
42 psi
wymiary złoża
7 mm × 25 mm
objętość złoża
1 mL
kolumna śr. wewn.
7 mm
rozmiar
śr. rozm. cząst.
34 μm
cleaning
5-10
Zakres roboczy
5-10
Powiązane kategorie
Opis ogólny
Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.
Heparin is coupled to the Sepharose™ High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap® Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap® Heparin HP columns equilibrated with 25 mL of 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily performed with a syringe and the supplied Luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4° C to 30° C.
Cechy i korzyści
- Heparin binds to a wide range of biomolecules via both affinity and ion exchange mechanisms.
- Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma proteins.
- Simple operation with a syringe, a pump or a chromatography system such as AKTA design
- Stable over pH 5 to 10 and tolerates all commonly used aqueous buffers.
Przechowywanie i stabilność
Komentarz do analizy
Inne uwagi
Informacje prawne
produkt powiązany
Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Kod klasy składowania
3 - Flammable liquids
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Produkty
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from GE Healthcare.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protokoły
Na tej stronie pokazano, jak oczyszczać lub usuwać białka wiążące DNA za pomocą Heparin Sepharose High Performance, Heparin Sepharose 6 Fast Flow, Capto Heparin firmy Cytiva.
This page shows how to purify or remove DNA-binding proteins with Heparin Sepharose High Performance, Heparin Sepharose 6 Fast Flow, Capto Heparin from GE Healthcare.
DNA binding proteins form an extremely diverse class of proteins sharing a single characteristic, their ability to bind to DNA. Functionally the group can be divided into those responsible for the replication and orientation of the DNA such as histones, nucleosomes and replicases and those involved in transcription such as RNA/DNA polymerases, transcriptional activators and repressors and restriction enzymes.
How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
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