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GE17-0973-05

Phenyl Sepharose™ 6 Fast Flow (High Sub)

Cytiva 17-0973-05, pack of 200 mL

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About This Item

Kod UNSPSC:
41106500
NACRES:
NA.56

ligand

phenyl

opakowanie

pack of 200 mL

producent / nazwa handlowa

Cytiva 17-0973-05

macierz

6% cross-linked agarose

wielkość cząstki

45-165 μm

średnia średnica

90 μm

cleaning in place

2-14

working range

3-13

przydatność

suitable for bioprocess medium

Powiązane kategorie

Opis ogólny

Phenyl Sepharose™ 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) medium for capture and intermediate purification requiring low to medium hydrophobicity.

Phenyl Sepharose™ 6 Fast Flow is part of the Sepharose™ Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.
Phenyl Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages. Two levels of ligand substitution degree help to find the optimal selectivity and binding capacity for a given application.

Phenyl Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Phenyl Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Cechy i korzyści

  • Standard aromatic HIC media based on Sepharose™ Fast Flow base matrix derivatized via uncharged, chemically-stable ether linkages.
  • Designed for initial and intermediate purification requiring low to medium hydrophobicity
  • Two levels of substitution help find optimal selectivity and binding capacity for a given application
  • BioProcess medium supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding.

Przechowywanie i stabilność

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Komentarz do analizy

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informacje prawne

Sepharose is a trademark of Cytiva
This page may contain text that has been machine translated.

Piktogramy

Flame
GHS02

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H226

Zwroty wskazujące środki ostrożności

P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501

Kod klasy składowania

3 - Flammable liquids

Certyfikaty analizy (CoA)

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Produkty

Wybór soli i przygotowanie buforu

Optymalizacja warunków solnych dla wiązania białek i przechodzenia zanieczyszczeń w chromatografii w celu osiągnięcia wymaganej selektywności.

Salt Selection and Buffer Preparation

This section is about salt selection and buffer preparation in HIC medium from GE Healthcare, parameters such as buffer ions and pH are considered

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography

HIC as a Capture Step

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

Zobacz wszystko

Protokoły

Selectivity and the Properties of a HIC Medium

While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent cleaning conditions and their low level of non-specific interaction.

HIC Purification Options and Scale up with SOURCE™ Media

This page discusses how to do a purification with SOURCE™ media from GE Healthcare

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

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