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F4383
7-Fluorobenzofurazan-4-sulfonic acid ammonium salt
≥98%
Synonim(y):
4-Fluoro-7-sulfobenzofurazan ammonium salt, 7-Fluorobenz-2,1,3-oxadiazole-4-sulfonic acid ammonium salt, Ammonium 7-fluorobenzofurazan-4-sulfonate, SBD-F
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About This Item
Wzór liniowy:
C6H3FN2O4S · NH3
Numer CAS:
Masa cząsteczkowa:
235.19
Beilstein:
5199564
Numer MDL:
Kod UNSPSC:
12352106
Identyfikator substancji w PubChem:
NACRES:
NA.32
Polecane produkty
Poziom jakości
Próba
≥98%
warunki przechowywania
protect from light
fluorescencja
λex 380 nm; λem 515 nm
temp. przechowywania
−20°C
ciąg SMILES
N.OS(=O)(=O)c1ccc(F)c2nonc12
InChI
1S/C6H3FN2O4S.H3N/c7-3-1-2-4(14(10,11)12)6-5(3)8-13-9-6;/h1-2H,(H,10,11,12);1H3
Klucz InChI
JXLHNMVSKXFWAO-UHFFFAOYSA-N
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Opis ogólny
7-Fluorobenzofurazan-4-sulfonic acid ammonium salt, also known as SBD-F, is a low molecular weight sensitive fluorescent probe. SBD-F reacts with sulfhydryl groups (disulfides do not react with SBD-F and must first be reduced to thiols, such as tributylphosphine) to produce highly fluorescent compounds. The rate of reaction of thiols with SBD-F gradually increases with increasing pH. High fluorescence intensities are observed at pH 2-12. SBD-F has an excitation range between 380-385nm and an emission range between 510-515nm.
Zastosowanie
SBD-F is suitable for the HPLC determination of biological thiols at the picomole level. SBD-F has been used in HPLC methods for measuring total plasma and serum homocysteine levels. It is also suitable to determine metallothionein in a tandem column HPLC method with an isocratic solvent system. SBD-F has been used for determining the position of disulfide linkages of cysteine residues in proteins and for detecting cysteine-containing peptides.
Fluorescent probe for thiols and pre-column labeling of biological thiols for HPLC
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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