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Kluczowe dokumenty
69898
Bromobimane
suitable for fluorescence, BioReagent, ≥95% (HPCE)
Synonim(y):
Monobromobimane
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About This Item
Wzór empiryczny (zapis Hilla):
C10H11BrN2O2
Numer CAS:
Masa cząsteczkowa:
271.11
Beilstein:
4430959
Numer MDL:
Kod UNSPSC:
12171500
Identyfikator substancji w PubChem:
NACRES:
NA.32
Polecane produkty
linia produktu
BioReagent
Poziom jakości
Próba
≥95% (HPCE)
Formularz
solid
mp
152-154 °C (lit.)
rozpuszczalność
DMF: soluble
DMSO: soluble
acetonitrile: soluble
methanol: soluble
fluorescencja
λex 390 nm; λem 478 nm in 0.1 M phosphate pH 7.5 (after derivatization with glutathione)
λex 398 nm
przydatność
suitable for fluorescence
temp. przechowywania
2-8°C
ciąg SMILES
CC1=C(C)C(=O)N2N1C(CBr)=C(C)C2=O
InChI
1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3
Klucz InChI
AHEWZZJEDQVLOP-UHFFFAOYSA-N
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Opis ogólny
Bromobimane, also called probe for thiols, is a fluorescent reagent activated upon a photolysis reaction. Bromobimane in solution reacts with small thiol groups (e.g., GSH) and with reactive protein thiol groups (e.g., hemoglobin). The reaction of Bromobimane with thiols is of second-order and dependent on pH. And, upon reacting with thiolate, it activates the water-soluble fluorescent product for detection.
Zastosowanie
Bromobimane is used for the determination of thiols by the HPLC method. It is suitable as a pre-column derivatization agent for fluorometric determination of 2,3-dimercaptopropane-1-sulfonic acid and other dithiols. Bromobimane has been used as a fluorescent label in studying oligomycin-sensitive ATPase from beef heart mitochondria.
Cechy i korzyści
The benefits of using Bromobimane for the analysis of low molecular weight thiols are: Highly selective.
- Rapid reactivity.
- Ease of separation of derivatives by reverse HPLC.
- Ability to penetrate cells.
Opakowanie
Bottomless glass bottle. Contents are inside inserted fused cone.
Inne uwagi
Fluorescent thiol-specific reagent
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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