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D8312

Sigma-Aldrich

REDTaq® Genomic DNA Polymerase

with MgCl2

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Wszystkie zdjęcia(3)

About This Item

Numer MDL:
MFCD01635818
Kod UNSPSC:
12352204
NACRES:
NA.55

Poziom jakości

200

Postać

liquid

zastosowanie

sufficient for 1000 reactions
 sufficient for 250 reactions
 sufficient for 2500 reactions

Właściwości

Difficult Templates/Specialty Enzymes PCR
dNTPs included: no
hotstart: no

stężenie

1 unit/μL

metody

PCR: suitable

kolor

red

moc wejściowa

purified DNA

Zastosowanie

agriculture

Warunki transportu

wet ice

temp. przechowywania

−20°C

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Opis ogólny

REDTaq® Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method.
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides (the building blocks of DNA). These enzymes are essential for DNA replication and usually work in pairs. It creates two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.

Zastosowanie

REDTaq® Genomic DNA Polymerase is used for routine PCR amplification of genomic DNA.

Cechy i korzyści

  • Enhanced amplification on genomic and difficult DNA templates
  • Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications
  • Quick recognition and confirmation of appropriate mixing
  • No loading buffers or tracking dyes necessary. Sample can be taken directly from reaction and loaded onto an agarose gel
  • The samples can be re-amplified as in nested PCR
  • The red dye migrates faster than bromophenol blue
  • Greater consistency across reactions due to proper mixing

Definicja jednostki

One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74°C.

Informacje prawne

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
This page may contain text that has been machine translated.

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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Preparation of genomic DNA from Dictyostelium discoideum for PCR analysis.
Steve J Charette et al.
BioTechniques, 36(4), 574-575 (2004-04-20)
J A F Demandt et al.
Scientific reports, 11(1), 425-425 (2021-01-13)
Hypoxia is prevalent in atherosclerotic plaques, promoting plaque aggravation and subsequent cardiovascular disease (CVD). Transmembrane protein carbonic anhydrase IX (CAIX) is hypoxia-induced and can be shed into the circulation as soluble CAIX (sCAIX). As plaque macrophages are hypoxic, we hypothesized a
L M Winton et al.
Phytopathology, 92(1), 112-116 (2008-10-24)
ABSTRACT Phaeocryptopus gaeumannii is a widespread foliar parasite of Douglas-fir. Although normally innocuous, the fungus also causes the defoliating disease Swiss needle cast in heavily infected needles. The extent of P. gaeumannii colonization in Douglas-fir foliage was estimated with real-time
Genotyping of HLA-B27 by real-time PCR without hybridization probes.
M A Bon et al.
Clinical chemistry, 46(7), 1000-1002 (2000-07-15)
Jill A Fahrner et al.
Cancer research, 62(24), 7213-7218 (2002-12-25)
We examined the relationship between aberrant DNA hypermethylation and key histone code components at a hypermethylated, silenced tumor suppressor gene promoter in human cancer. In lower eukaryotes, methylated H3-lysine 9 (methyl-H3-K9) determines DNA methylation and correlates with repressed gene transcription.
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Protokoły

Applications with REDTaq™

Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

Amplification of Genomic DNA using REDTaq DNA Polymerase

Method for PCR using genomic DNA or other complex templates

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