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D2812
REDTaq® Genomic DNA Polymerase
without MgCl2
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About This Item
Polecane produkty
Poziom jakości
Formularz
liquid
zastosowanie
sufficient for 1000 reactions
sufficient for 250 reactions
sufficient for 2500 reactions
Właściwości
Difficult Templates/Specialty Enzymes PCR
dNTPs included: no
hotstart: no
stężenie
1 unit/μL
metody
PCR: suitable
kolor
red
moc wejściowa
purified DNA
Zastosowanie
agriculture
Warunki transportu
wet ice
temp. przechowywania
−20°C
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Opis ogólny
REDTaq® Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method.
Zastosowanie
REDTaq® Genomic DNA Polymerase has been used as a component of the polymerase chain reaction (PCR) mix for PCR amplification. It has also been used as a component of the preamplification mix for PCR in amplified fragment length polymorphism (AFLP) analysis of Fusarium oxysporum sp. isolates.
Cechy i korzyści
- Enhanced amplification on genomic and difficult DNA templates
- Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications
- Quick recognition and confirmation of appropriate mixing
- No loading buffers or tracking dyes necessary. Sample can be taken directly from reaction and loaded onto an agarose gel
- The samples can be re-amplified as in nested PCR
- The red dye migrates faster than bromophenol blue
- Greater consistency across reactions due to proper mixing
Komponenty
Includes 10× PCR reaction buffer without MgCl2 and a separate tube of 25 mM MgCl2
Definicja jednostki
One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74°C.
Informacje prawne
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
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Cennik
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Resp. Sens. 1
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
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Protokoły
Method for PCR using genomic DNA or other complex templates
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