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D1806

Sigma-Aldrich

Taq DNA Polymerase from Thermus aquaticus

with 10× PCR reaction buffer containing MgCl2

Synonim(y):

MgCl2 taq polymerase, Taq polymerase

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About This Item

Numer CAS:
9012-90-2
Numer EC enzymu:
2.7.7.7 (BRENDA, IUBMB)
Numer MDL:
MFCD00166026
Kod UNSPSC:
12352204
NACRES:
NA.55

pochodzenie biologiczne

enzyme from bacterial (Thermus Aquaticus)

Poziom jakości

200

rekombinowane

expressed in E. coli

Postać

liquid

zastosowanie

sufficient for 10000 reactions
 sufficient for 3000 reactions
 sufficient for 500 reactions

masa cząsteczkowa

94 kDa

Właściwości

dNTPs included: no
hotstart: no

stężenie

5 units/μL

metody

PCR: suitable

kolor

colorless

moc wejściowa

purified DNA

przydatność

suitable for PCR and automated sequencing reactions

Zastosowanie

agriculture

Warunki transportu

wet ice

temp. przechowywania

−20°C

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Opis ogólny

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. The enzyme is in a recombinant form, expressed in E. coli. It can withstand repeated heating to 95 °C without significant loss of activity. Each lot of Taq DNA Polymerase is tested for PCR amplification and double-stranded sequencing.

Zastosowanie

Taq DNA Polymerase from Thermus aquaticus has been used:
  • in the quantification of fungal growth by polymerase chain reaction (PCR) and photometric assay
  • in conventional reverse transcriptase (RT)-PCR
  • in simple sequence repeats (SSR) genotyping
  • as a component of PCR mix for amplification of genomic and mitochondrial DNA
  • in direct tetra-primer amplification refractory mutation system (T-ARMS) PCR to amplify dried whole blood samples

Działania biochem./fizjol.

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.

Cechy i korzyści

  • Low per unit cost of Taq

Opakowanie

Taq DNA Polymerase with 10× reaction buffer containing MgCl2
Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl2 (D1806) or a 10× reaction buffer without MgCl2 plus a separate tube of MgCl2 for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification.

Inne uwagi

Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity.

Definicja jednostki

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Informacje prawne

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
This page may contain text that has been machine translated.

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Kod klasy składowania

12 - Non Combustible Liquids

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Odwiedź Bibliotekę dokumentów

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Human endogenous retrovirus family HERV-K (HML-2) RNA transcripts are selectively packaged into retroviral particles produced by the human germ cell tumor line Tera-1 and originate mainly from a provirus on chromosome 22q11. 21
Ruprecht K, et al.
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Produkty

Introduction and Historical Timelines

Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.

Protokoły

dNTP Mediated Hot Start PCR Protocol

Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.

Standardowy protokół PCR

Poznaj standardowe kroki protokołu PCR i przejrzyj listy odczynników lub parametry cykli. Ta metoda rutynowej amplifikacji DNA metodą PCR wykorzystuje standardową polimerazę Taq DNA.

Standard PCR Protocol

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

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