CORET

PCR Core kit with Taq DNA polymerase

PCR components in separate tubes to allow for optimization

• Synonim(y): PCR kit, Taq DNA Polymerase kit
• Kod UNSPSC: 41106300
• eCl@ss: 32161000
• NACRES: NA.55

Właściwości

• Poziom jakości: 200
• Formularz: liquid
• zastosowanie: sufficient for 250 reactions
• Właściwości: dNTPs included; hotstart: no
• metody: PCR: suitable
• kolor: colorless
• moc wejściowa: purified DNA
• Warunki transportu: wet ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. This enzyme is in a recombinant form, expressed in E. coli. Taq DNA polymerase enzyme can withstand high temperatures up to 95 °C without significant loss of activity. Taq DNA Polymerase is a magnesium ion-dependent enzyme. Taq DNA Polymerase possesses 5′→3′ DNA polymerase activity and 5′→3′ exonuclease activity.

Zastosowanie

CORET PCR kit has been used for the amplification of genomic DNA during the study of genetic diversity in various species of Manilkara zapota.

PCR Core kit with Taq DNA polymerase is suitable for the amplification of genomic DNA, cDNA, or cloned DNA templates. Each component has been tested in the polymerase chain reaction. Optimal concentrations of Taq polymerase, template DNA, primers, and MgCl₂ will depend on the specifics of the system. It may be necessary to optimize each component separately, especially the Taq and Mg²⁺ concentrations and the cycling parameters.

Ilość

Kit contains enough reagents for 250 units of Taq DNA polymerase.

Inne uwagi

The following reagents are required but not provided in the kit:

• Mineral Oil, M 8662 (optional)
• Thermocycler
• Primers
• DNA to be amplified
• Chloroform, C 7559 (optional)

Informacje prawne

No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

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Elementy zestawu są też dostępne oddzielnie

• P2192 10X PCR Buffer, Optimized for routine PCR with MgCl₂ included 1 mL/vial
• P2317 10X PCR Buffer without MgCl₂, Optimized for routine PCR without MgCl₂ 1 mL/vial
• M8787 Magnesium chloride solution, PCR Reagent, 25 mM MgCI₂ solution for PCR 1 mL/vial
• W1754 Water, PCR Reagent 3 vial(s)
• D7295 Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .25 mL

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable