Przejdź do zawartości
Merck
Wszystkie zdjęcia(1)

Key Documents

Inne dokumenty

P2192

Sigma-Aldrich

10X PCR Buffer

Optimized for routine PCR with MgCl2 included

Synonim(y):

PCR Buffer Solution, PCR Reaction Buffer

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wszystkie zdjęcia(1)

About This Item

Kod UNSPSC:
41106306
NACRES:
NA.52

Poziom jakości

300

Postać

liquid

opakowanie

vial of 1.5 mL

kolor

colorless

Zastosowanie

agriculture

obecność zanieczyszczeń

DNase, RNase, none detected

temp. przechowywania

−20°C

Zastosowanie

10X PCR Buffer has been used as a component of the PCR mixture:
  • for the amplification of viral DNA
  • to amplify genomic DNA to study the methylenetetrahydrofolate reductase (MTHFR) gene mutations in attention deficit hyperactivity disorder (ADHD) individuals
  • to amplify 16S-23S rRNA gene internal transcribed spacer (ITS) from Bacillus sp.

Cechy i korzyści

  • Standalone buffer
  • Compatible with JumpStart™ Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq® Genomic DNA Polymerase (D8312)

Komponenty

Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl2; 0.01% gelatin

Informacje prawne

JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
This page may contain text that has been machine translated.

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Maike Stam et al.
Genetics, 162(2), 917-930 (2002-10-26)
Paramutation is an interaction between alleles that leads to a heritable change in the expression of one allele. In B'/B-I plants, B-I (high transcription) always changes to B' (low transcription). The new B' allele retains the low expression state in
G J Breedveld et al.
Neurology, 59(4), 579-584 (2002-08-28)
Benign hereditary chorea (BHC) is an autosomal dominant disorder that can be distinguished from Huntington disease by its early onset, stable or only slightly progressive course, and absence of mental deterioration. The variation in clinical features is such that its
Ákos Boros et al.
PloS one, 6(12), e29145-e29145 (2011-12-24)
A novel positive-sense, single-stranded RNA (+ssRNA) virus (Halastavi árva RNA virus, HalV; JN000306) with di-cistronic genome organization was serendipitously identified in intestinal contents of freshwater carps (Cyprinus carpio) fished by line-fishing from fishpond "Lőrinte halastó" located in Veszprém County, Hungary.
Cem Gokcen et al.
International journal of medical sciences, 8(7), 523-528 (2011-09-08)
The purpose of this study was to evaluate the relationship between 5,10- methylenetetrahydrofolate reductase (MTHFR) polymorphisms and Attention Deficit Hyperactivity Disorder (ADHD) in a sample of Turkish children. MTHFR gene polymorphisms were assessed in 40 patients with ADHD and 30
Zhong-Dong Shi et al.
Stem cells translational medicine, 9(11), 1378-1388 (2020-07-15)
Human pluripotent stem cells, including induced pluripotent stem cells (iPSCs) and embryonic stem cells, hold great promise for cell-based therapies, but safety concerns that complicate consideration for routine clinical use remain. Installing a "safety switch" based on the inducible caspase-9
Wyświetl wszystkie powiązane dokumenty

Protokoły

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej