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M1028

Sigma-Aldrich

Magnesium chloride solution

for molecular biology, 1.00 M±0.01 M

Synonim(y):

MgCl2, dichloromangesium, magnesium chloride

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About This Item

Wzór liniowy:
MgCl2
Numer CAS:
7786-30-3
Masa cząsteczkowa:
95.21
Numer MDL:
MFCD00011106
Kod UNSPSC:
12352302
Identyfikator substancji w PubChem:
24896582
NACRES:
NA.52

klasa czystości

for molecular biology

Poziom jakości

300

sterylność

non-sterile; 0.2 μm filtered

Postać

liquid

stężenie

1.00 M±0.01 M

metody

DNA sequencing: suitable

zanieczyszczenia

DNAse, none detected
 NICKase, none detected
 Protease, none detected
 RNAse, none detected

rozpuszczalność

water: soluble

gęstość

1.080 g/cm3

ślady kationów

heavy metals (as Pb): ≤5 ppm

przydatność

suitable for electrophoresis
suitable for molecular biology

Zastosowanie

cell analysis

obecność zanieczyszczeń

DNase, RNase, NICKase and protease, none detected

ciąg SMILES

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

Klucz InChI

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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Opis ogólny

Magnesium chloride is a common ionic halide that readily dissolves in water. It plays various crucial biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid composition. Additionally, magnesium chloride serves as an essential co-factor in numerous enzymes, including DNAse, specific restriction enzymes, and Ribonuclease H. It is widely utilized to provide magnesium ions in diverse molecular biology applications, including PCR reactions.

Zastosowanie

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions and buffers. It has been used -
  • to prepare buffer to lyse cells for immunoprecipitation
  • as a supplement in Krebs-Henseleit buffer in chronic high-fat diet studies of aged mice.
  • in embedding 3-dimensional mtgase hydrogels in invitro studies of osteocyte differentitaion and interconnected cellular networks.
  • It has been used to protect the liver from oxidative damage.
  • to prepare serum-free medium for electrophysiological studies of rat brain slice cultures.

Cechy i korzyści

  • Available as a Ready-to-use solution which reduces the need for preparation time.
  • Heavy metals (as Pb): ≤5 ppm
  • Free from DNase, RNase, NICKase and protease

Komponenty

Magnesium chloride solution contains 1M magnesium chloride in 18 megaohm water.

Inne uwagi

For additional information on our range of Biochemicals, please complete this form.
This page may contain text that has been machine translated.

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Antimo Migliaccio et al.
Methods in molecular biology (Clifton, N.J.), 776, 361-370 (2011-07-29)
Much evidence indicates that, with few exceptions, non-genomic actions of steroids are mediated by receptors universally known as nuclear receptors. Steroid receptors do not exhibit intrinsic tyrosine kinase activity. Nevertheless, they stimulate different signaling pathways in cytoplasm of target cells
Yelena Y Grinberg et al.
Journal of neurochemistry, 122(1), 221-229 (2012-04-25)
Spreading depression (SD), the likely cause of migraine aura and perhaps migraine, is triggered by widespread and unfettered neuronal hyperexcitability. Migraine and the initiating hyperexcitability of seizure, which involve oxidative stress (OS), are likely interrelated. Environmental enrichment (EE) decreases seizure
Bert De Rybel et al.
Science (New York, N.Y.), 345(6197), 1255215-1255215 (2014-08-12)
Coordination of cell division and pattern formation is central to tissue and organ development, particularly in plants where walls prevent cell migration. Auxin and cytokinin are both critical for division and patterning, but it is unknown how these hormones converge
Elaine T Lim et al.
PLoS genetics, 10(7), e1004494-e1004494 (2014-08-01)
Exome sequencing studies in complex diseases are challenged by the allelic heterogeneity, large number and modest effect sizes of associated variants on disease risk and the presence of large numbers of neutral variants, even in phenotypically relevant genes. Isolated populations
Hideyuki Arita et al.
Brain tumor pathology, 32(1), 22-30 (2014-04-22)
Assessment of the mutational status of the isocitrate dehydrogenase 1/2 (IDH1/2) gene has become an integral part of the standard diagnostic procedure and, therefore, needs to be accurate. This may, however, be compromised by various factors including the method of
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Produkty

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Method outlines use of a hot start Taq for multiplex qPCR and provides guidance on how to optimize dNTPs, primer, probes and MgCL2 concentrations. By optimizing these parameters, the user can improve assay sensitivity and linear range of detection.

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