P19 Cell Line from mouse

95102107, mouse embryo, Not specified

• Kod UNSPSC: 41106514
• pochodzenie biologiczne: mouse embryo
• tryb wzrostu: Adherent
• kariotyp: Euploid
• morfologia: Not specified
• produkty: Not specified
• receptory: Not specified
• powiązane choroby: cancer

Właściwości

• Nazwa produktu: P19 Cell Line from mouse, 95102107
• pochodzenie biologiczne: mouse embryo
• opis: Mouse teratocarcinoma
• tryb wzrostu: Adherent
• kariotyp: Euploid
• morfologia: Not specified
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Mouse teratocarcinoma

Opis linii komórkowej

The P19 cell line was derived from an embryonal carcinoma induced in a C3H/He strain mouse. The cells can be cloned at high efficiency in medium containing 0.1mM β-mercaptoethanol. The pluripotent P19 cells can be induced to differentiate into neuronal and glial cells in the presence of retinoic acid. Aggregates of P19 cells differentiate into cardiac and skeletal muscle in the presence of dimethyl sulfoxide (DMSO). In the presence of both retinoic acid and DMSO cells develop as if exposed to retinoic acid only.

Zastosowanie

Differentiation studies

pożywka hodowlana

α MEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2.5% Foetal Bovine Serum (FBS) + 7.5% Calf Serum (CS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Ta strona może zawierać tekst przetłumaczony maszynowo.