WEHI 164 Cell Line from mouse

87022501, mouse skin (fibroblast), Fibroblast

• Kod UNSPSC: 41106514

Właściwości

• Nazwa produktu: WEHI 164 Cell Line from mouse, from mouse skin(fibroblast), 87022501
• pochodzenie biologiczne: mouse skin (fibroblast)
• tryb wzrostu: Adherent
• kariotyp: Not specified
• morfologia: Fibroblast
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Mouse BALB/c fibrosarcoma

Opis linii komórkowej

Established from a fibrosarcoma induced by subcutaneous injection of methylcholanthrene in BALB/c mice. Highly sensitive after pre-treatment with actinomycin D to human cytotoxic monocytes, to tumour necrosis factor and to lymphotoxin. It provides convenient target cells for short-term (51) chromium release assays.

Zastosowanie

Tumourigenicity and cytotoxicity studies, chromium release assays

pożywka hodowlana

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Ta strona może zawierać tekst przetłumaczony maszynowo.