CAD cell line from mouse

8100805, mouse nerve, Neuronal

• Synonim(y): CAD Cell Line
• Kod UNSPSC: 41106514

Właściwości

• product name: CAD cell line from mouse, 08100805
• pochodzenie biologiczne: mouse nerve
• opis: Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumor
• tryb wzrostu: Adherent
• kariotyp: Not specified
• morfologia: Neuronal
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumour

Opis linii komórkowej

CAD cells (Cath.-a-differentiated) are a variant of a CNS catecholaminergic cell line established from a brain tumour in a transgenic mouse carrying wild-type SV40 T antigen under transcriptional control of rat tyrosine hydroxylase promoter. CAD has lost the immortalising oncogene and expresses neurone-specific proteins and synaptic vesicle proteins. CAD will differentiate to form neuronal processes in serum free conditions.

Zastosowanie

Study of neuronal differentiation

pożywka hodowlana

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 8% Foetal Bovine Serum (FBS)

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 X 10⁴ cells/cm² Cells should be dislodged by gentle pipetting; 5% CO₂; 37°C.

Inne uwagi

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