CLC

95070628, fish blood, Epithelial-like

• Kod UNSPSC: 41106514

Właściwości

• product name: CLC, 95070628
• pochodzenie biologiczne: fish blood
• tryb wzrostu: Adherent
• kariotyp: 2n = 104
• morfologia: Epithelial-like
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Carp leukocyte

Opis linii komórkowej

The cell line CLC (Carp Leukocyte Culture) was established from peripheral blood mononuclear cells of a normal 2 year old female common carp, Cyprinus carpio. They possess phagocytic activity and are reported to be stimulated by TNF, gamma-interferon and LPS therefore resembling characteristic features of monocytes / macrophages. CLC replicate fish viruses e.g. IPNV, PFR, SVCV and VHSV.

Zastosowanie

Immunological research, fish virology, fish cell physiology.

pożywka hodowlana

EMEM (EBSS) or TC-199 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Spilt confluent cultures 1:3 using 0.25% trypsin or trypsin/EDTA; 25-28°C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.

Inne uwagi

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This page may contain text that has been machine translated.