SK-N-BE(2)

95011815, human nerve, Epitheilioid & neuroblastoid

• Kod UNSPSC: 41106514

Właściwości

• product name: SK-N-BE(2), 95011815
• pochodzenie biologiczne: human nerve
• tryb wzrostu: Adherent
• kariotyp: Modal no. 44
• morfologia: Epitheilioid & neuroblastoid
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: metastasis
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian neuroblastoma

Opis linii komórkowej

Established from a bone marrow biopsy of a 22-month-old male with disseminated neuroblastoma in 1972. The morphology varies with sub-population from cells with long processes through small, flattened, epithelioid cells. Dense cultures tend to form loosely adherent aggregates. They are multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine.

Zastosowanie

Chemotherapy development, chromosome studies, drug resistance, differentiation studies.

pożywka hodowlana

EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) : Ham′s F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:10 to 1:25 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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