IPI-2I

93100622, Porcine intestine, Epithelial-like

• Kod UNSPSC: 41106514

Właściwości

• Nazwa produktu: IPI-2I, 93100622
• pochodzenie biologiczne: Porcine intestine
• tryb wzrostu: Adherent
• kariotyp: Heteroploid
• morfologia: Epithelial-like
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Boar Miniature male ileum, SV40 transformed

Opis linii komórkowej

Epitheloid cell line derived from the ileum of an adult histocompatible miniature male boar. Transformed by SV40 plasmid pSV3-neo. Electron microscopy analysis shows evidence of microvilli and desmosomes connecting cells. The cells were found to co-express cytokeratin 18 specific of simple luminal epithelial cells and vimentin associated with a mesenchymal origin. Susceptible to Transmissible Gastro-enteritis (TG) virus.

Zastosowanie

Studies of epithelial cell differentiation.

pożywka hodowlana

DMEM + 4mM Glutamine + 0.024IU/ml Insulin + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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