C6 Cell Line from rat

92090409, rat nerve (glial tumor), Neuronal

• Synonim(y): C-6 Cells, RGC-6 Cells
• Kod UNSPSC: 41106514

Właściwości

• Nazwa produktu: C6 Cell Line from rat, 92090409
• pochodzenie biologiczne: rat nerve (glial tumor)
• opis: Rat glial tumor
• tryb wzrostu: Adherent
• kariotyp: 2n = 42
• morfologia: Neuronal
• produkty: S-100 protein, somatotrophin
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Rat glial tumour

Opis linii komórkowej

Derived from a rat glial tumour induced by N-nitrosomethylurea. The cells produce S-100 protein which is found in the neural tissue of vertebrates and has been found in brain tumours. Synthesis of S-100 increases 10 fold as cells reach confluency.

Zastosowanie

C6 cell line has been used to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions. It has also been used to study the cytotoxic and antioxidant properties of supercritical CO₂ extract (SCE) of old man′s beard (Usnea barbata).

C6 cells are reported to produce elevated levels of glyceryl phosphate dehydrogenase activity in response to glucocorticoids

pożywka hodowlana

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x100,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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