GS-109-V-20

90110505, human skin, Fibroblast-like

• Kod UNSPSC: 41106514

Właściwości

• product name: GS-109-V-20, 90110505
• pochodzenie biologiczne: human skin
• tryb wzrostu: Adherent
• kariotyp: 2n = 46
• morfologia: Fibroblast-like
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian skin fibroblast Gardner′s syndrome

Opis linii komórkowej

Derived from skin from the upper arm of an 18 year old Caucasian male with Gardner′s syndrome is an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. Cells exist in an initiated state rather than a normal or transformed state. Normal diploid karyotype.

Zastosowanie

Tumourigenicity studies

pożywka hodowlana

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Monolayer will form in a 25cm² flask in 1 week.

Inne uwagi

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