NB4 1A3

89121405, mouse nerve, Neuroblast

• Kod UNSPSC: 41106514

Właściwości

• product name: NB4 1A3, 89121405
• pochodzenie biologiczne: mouse nerve
• tryb wzrostu: Adherent
• kariotyp: Unstable
• morfologia: Neuroblast
• produkty: Acetylcholinesterase, choline acetylase, tyrosine hydroxylase
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Mouse C-1300 Neuroblastoma

Opis linii komórkowej

Cloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternate in vivo and in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses.

Zastosowanie

Tumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virus

pożywka hodowlana

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Inne uwagi

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