HL60 Ast.4

88120802, human blood, Promyeloid, blast/promyelocyte

• Kod UNSPSC: 41106514

Właściwości

• product name: HL60 Ast.4, 88120802
• pochodzenie biologiczne: human blood
• tryb wzrostu: Suspension
• kariotyp: 2n = 48, hyperdiploid
• morfologia: Promyeloid, blast/promyelocyte
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian promyelocytic leukaemia

Opis linii komórkowej

Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Treatment with 50nM TPA results in only very limited differentiation towards basophilic morphology (Giemsa stain). Differentiation towards monocyte lineage has not been observed.

Zastosowanie

Differentiation studies

pożywka hodowlana

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).

Rutyna subkultury

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Do not allow the culture medium to become acidic.

Inne uwagi

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