HL60 Ast.3

88120801, human blood, Lymphoblastoid

• Kod UNSPSC: 41106514

Właściwości

• product name: HL60 Ast.3, 88120801
• pochodzenie biologiczne: human blood
• tryb wzrostu: Suspension
• kariotyp: Modal no. = 93, Hypertetraploid
• morfologia: Lymphoblastoid
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian promyelocytic leukaemia

Opis linii komórkowej

Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Cells are capable of differentiating into neutrophils by induction with 1.75% DMSO.

Zastosowanie

Differentiation studies

Profil DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 7
TPOX: 8,11
vWA: 16

pożywka hodowlana

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).

Rutyna subkultury

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Do not allow the culture medium to become acidic or the cell line will differentiate.

Inne uwagi

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