TA3 Hauschka

85061102, mouse breast, Epithelial

• Kod UNSPSC: 41106514

Właściwości

• product name: TA3 Hauschka, 85061102
• pochodzenie biologiczne: mouse breast
• tryb wzrostu: Adherent
• kariotyp: 2n = 41
• morfologia: Epithelial
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Mouse mammary carcinoma

Opis linii komórkowej

In form of tumorigenic ascites shows diminished expression of histocompatibility antigens and readily overcomes histoincompatibility barriers. Immunoresistant TA3/Ha sub-line arose spontaneously without subjection to any known immunological selection pressure.

Zastosowanie

Tumourigenicity studies

pożywka hodowlana

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells grow partly in suspension, therefore spin down supernatant at time of subculture to collect the cells.

Inne uwagi

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