Przejdź do zawartości
Merck
Wszystkie zdjęcia(1)

Kluczowe dokumenty

C-12901

Sigma-Aldrich

Human Mononuclear Cells (hMNC)

Isolated from cord blood, single donor, ultra-pure, 25 million cryopreserved cells

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
41106514
NACRES:
NA.81

pochodzenie biologiczne

human umbilical cord blood

opakowanie

pkg of 25,000,000 cells

morfologia

(mononuclear)

metody

cell culture | mammalian: suitable

Warunki transportu

dry ice

temp. przechowywania

−196°C

Opis ogólny

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Pochodzenie linii komórkowej

Cord Blood

Zastosowanie

Mononuclear Cells (MNC) represent the lymphocyte and monocyte fraction isolated from umbilical cord blood. They are prepared by optimized low-density gradient centrifugation, which effectively reduces the granulocyte content. Red blood cells are gently but thoroughly depleted by proprietary techniques instead of osmotic lysis. This results in ultra-pure mononuclear cells with superior viability and unchanged biological function. In addition, the cells do not clump after thawing, allowing for easy handling and reproducible results.The MNC can be used directly for experiments or as a starting material for the isolation of several cell types, e.g., hematopoietic and endothelial progenitors, monocytes, and lymphocyte subpopulations.

Jakość

Rigid quality control tests are performed for each lot of Mononuclear Cells. They are routinely characterized by flow cytometry analyzing a series of cellular parameters, e.g. viability, cell size, granularity. provides detailed information on the percentages of the major cellular sub-populations, i.e., the lymphocytes, the monocytes, and granulocytes, for each lot of Mononuclear Cells. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Ostrzeżenie

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Rutyna subkultury

Click here for more information.

Inne uwagi

Recommended Plating Density: 1 x 106 cells per mlTested Markers: CD31 negative, CD34 negative, CD105 positive, CD146 positive

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Ta strona może zawierać tekst przetłumaczony maszynowo.

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Dokumenty section.

Proszę o kontakt, jeśli potrzebna jest pomoc Obsługa Klienta

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Produkty

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

Odkryj ponad 350 produktów PromoCell na Sigma.com, w tym kriokonserwowane komórki krwi i pożywki do hodowli komórkowych dla różnych komórek ludzkich.

Protokoły

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej