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C-12921

Sigma-Aldrich

Human CD34+ Progenitor Cells (hCD34+-CB)

Isolated from cord blood, single donor, 100,000 cryopreserved cells

Synonim(y):

hCD34+-CB cells

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About This Item

Kod UNSPSC:
41106514

pochodzenie biologiczne

human umbilical cord blood

opakowanie

pkg of 100,000 cells

morfologia

(progenitor)

metody

cell culture | mammalian: suitable

Warunki transportu

dry ice

temp. przechowywania

−196°C

Powiązane kategorie

Opis ogólny

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Pochodzenie linii komórkowej

Cord Blood

Zastosowanie

CD34 is a glycosylated transmembrane protein and represents a well-known marker for primitive blood- and bone marrow-derived progenitor cells, especially for hematopoietic and endothelial stem cells. Although the biological functions of CD34 are largely unknown, recent data suggest that CD34 is involved in maintenance of the progenitor cells in a phenotypically undifferentiated state. PromoCell offers CD34+ Progenitor Cells from the cord blood of healthy donors. The CD34+ Progenitor Cells contain two main cellular subpopulations, hematopoietic and endothelial progenitor cells. Therefore, CD34+ Progenitor Cells are suitable for a series of studies, e.g. directed differentiation into more committed types of blood cells and endothelial lineages.

Jakość

Rigid quality control tests are performed for each lot of CD34+ Progenitor Cells. They are routinely characterized by flow cytometry analyzing the viability, cell size, and the presence of significant markers, i.e. CD34. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Ostrzeżenie

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Rutyna subkultury

Click here for more information.

Inne uwagi

Recommended Plating Density: 5000 cells per mlTested Markers: CD34 positive

Polecane produkty

Recommended Primary Cell Culture Media:Link

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Produkty

Komórki progenitorowe CD34+ nadają się do różnorodnych badań, a także do ukierunkowanego różnicowania w bardziej zaangażowane fenotypy komórek krwi i linie śródbłonka.

Human Hematopoietic Progenitor Cells (HPC) and Growth Media

Przegląd komórek pierwotnych i mediów wykorzystywanych w hodowlach biomedycznych podkreśla różnice w stosunku do linii komórkowych.

Protokoły

In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products

In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products

Human CD34+ progenitor cells and xeno-free, defined media for the expansion of hematopoietic progenitor cells. Protocols for handling, cell passaging, media, and product use.

Step-by-step hematopoietic stem cell culture protocols for isolation, expansion and differentiation of CD34+ hematopoietic progenitor cells including CFU assays. It can be cultured under defined conditions designed either to promote self-renewal and increase the number of primitive cells.

Zobacz wszystko

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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