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Key Documents

SCC009

Sigma-Aldrich

ReNcell CX Human Neural Progenitor Cell Line & Culture Media Kit

ReNcell CX Kit contains human neural progenitor cells, optimized maintenance media & freezing media for culture of human neural stem cells.

Synonim(y):

Human NSC Line, Human Neural Stem Cell Line, Human Neural Stem Cells

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About This Item

Kod UNSPSC:
41106509
eCl@ss:
32011203
NACRES:
NA.75

pochodzenie biologiczne

human

Poziom jakości

producent / nazwa handlowa

Chemicon®
ReNcell

metody

cell culture | stem cell: suitable

moc wejściowa

sample type neural stem cell(s)

Warunki transportu

liquid nitrogen

Opis ogólny

ReNcell CX Kit contains human neural progenitor cells, optimized maintenance media and freezing media for culture of human neural stem cells. ReNcell CX Immortalized Cells (SCC007): ReNcell CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. ReNcell CX was derived from the cortical region of human fetal brain. Immortalized by retroviral transduction with the c-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the ReNcell CX retains a normal diploid karyotype in culture even after prolonged passage (>45 passages). ReNcell CX was developed by the ReNeuron Group plc, a biotech company that specializes in using human somatic stem cells for therapeutics. ReNcell CX may be used for a variety of research applications such as studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions. ReNcell CX cells have been obtained in a legal and ethical manner, compliant with current local informed consent procedures. ReNcell NSC Maintenance Medium (SCM005): ReNcell Neural Stem Cell (NSC) Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of ReNcell immortalized human neural progenitor cells. When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of ReNcell immortalized VM and CX neural stem cells. Withdrawal of the growth factors from ReNcell NSC Maintenance Medium will result in the spontaneous differentiation of ReNcell immortalized neural progenitor cells. ReNcell NSC Freezing Medium (SCM007): ReNcell NSC Freezing Medium is qualified for use with ReNcell immortalized human neural progenitor cell lines, CX (CHEMICON Catalog No. SCC007) and VM (CHEMICON Catalog No. SCC008) cultured in serum-free conditions with ReNcell NSC Maintenance Medium (CHEMICON Catalog No. SCM005). The optimized formulation allows for consistent cryopreservation and high viability upon thawing and plating.

Opis linii komórkowej

Neural Stem Cells

Zastosowanie

Research Category
Stem Cell Research

Komponenty

ReNcell Neural Stem Cell Freezing Medium (Cat.# SCM007)

ReNcell NSC Maintenance Media (Cat.# SCM005)

ReNcell CX Immortalized Cell Line (Cat.# SCC007)

Jakość

Each lot of ReNcell CX cells has been validated for high level of expression of Nestin and Sox 2 and for their self-renewal and multi-lineage differentiation capacities (please refer to datasheet figures). Cells also display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma. Maternal blood has been screened for HIV, HTLV, Hepatitis B and C.

Inne uwagi

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the "Academic Use Agreement" as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Kod klasy składowania

10 - Combustible liquids


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Differential development of neuronal physiological responsiveness in two human neural stem cell lines.
Donato, R; Miljan, EA; Hines, SJ; Aouabdi, S; Pollock, K; Patel, S; Edwards, FA; Sinden, JD
BMC Neuroscience null

Produkty

Zapoznaj się z rodzajami komórek macierzystych, w tym multipotencjalnymi komórkami macierzystymi, pluripotencjalnymi komórkami macierzystymi i iPSC oraz ich zastosowaniami w podstawowych badaniach nad komórkami macierzystymi, terapii komórkami macierzystymi i modelowaniu chorób.

Protokoły

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

Protokół hodowli komórek macierzystych do generowania modeli 3D NSC choroby Alzheimera z wykorzystaniem linii ludzkich neuronalnych komórek macierzystych ReNcell.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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