B2904

Bluo-Gal

reagent for selection of recombinant bacterial clones

• Synonim(y): 5-Bromo-3-indolyl β-D-galactopyranoside, substrate for β-galactosidase, Bluo-Gal
• Wzór empiryczny (zapis Hilla): C₁₄H₁₆BrNO₆
• Numer CAS: 97753-82-7
• Masa cząsteczkowa: 374.18
• Beilstein: 1550140
• Numer MDL: MFCD00063691
• Kod UNSPSC: 12352200
• Identyfikator substancji w PubChem: 24891417
• NACRES: NA.85

Właściwości

• klasa czystości: for molecular biology
• Poziom jakości: 200
• sterylność: non-sterile
• Próba: ≥98% (HPLC)
• Postać: powder
• metody: nucleic acid detection: suitable
• rozpuszczalność: DMF: soluble
• przydatność: suitable for substrate for β-galactosidase
• temp. przechowywania: −20°C
• ciąg SMILES: OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
• Klucz InChI: LINMATFDVHBYOS-MBJXGIAVSA-N

Opis

Opis ogólny

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Zastosowanie

Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac⁺ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.

Chromogenic substrate suitable for identification of lac⁺ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

Zasada

When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)