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B6650

Sigma-Aldrich

X-GlcA

reagent for selection of recombinant bacterial clones

Synonim(y):

5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, X-GlcA, X-glucuronide CHA salt

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About This Item

Wzór empiryczny (zapis Hilla):
C14H13BrClNO7 · C6H13N
Numer CAS:
114162-64-0
Masa cząsteczkowa:
521.79
Numer MDL:
MFCD00058543
Kod UNSPSC:
12352200
Identyfikator substancji w PubChem:
24891926
NACRES:
NA.85

klasa czystości

Molecular Biology Reagent
for molecular biology

Poziom jakości

200

sterylność

non-sterile

Próba

≥98% (TLC)

Postać

powder

metody

nucleic acid detection: suitable

rozpuszczalność

DMF: soluble

przydatność

suitable for β-galactosidase test

temp. przechowywania

−20°C

ciąg SMILES

NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34

InChI

1S/C14H13BrClNO7.C6H13N/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2/t9-,10-,11+,12-,14+;/m0./s1

Klucz InChI

JXCKZXHCJOVIAV-CYRSAHDMSA-N

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Opis ogólny

X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Zastosowanie

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.
X-GlcA has been used in histochemical staining of root sections for light microscopic observation.
Chromogenic substrate for β-glucuronidase (GUS) gene detection.

Zasada

When X-GlcA is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GlcA turn an intense blue color, allowing for easy differentiation between lac+ and lac- colonies.
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Sigma-Aldrich

Sigma-Aldrich

GUSS

β-Glucuronidase Reporter Gene Staining Kit

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