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GUSS
β-Glucuronidase Reporter Gene Staining Kit
Synonim(y):
β-Glucuronidase Staining Kit, Reporter Gene Kit, Staining Kit for Glucuronidase
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About This Item
Kod UNSPSC:
12161503
NACRES:
NA.32
Polecane produkty
zastosowanie
kit sufficient for 100 histochemical assays
Poziom jakości
metody
microbe id | staining: suitable
Warunki transportu
dry ice
temp. przechowywania
−20°C
Opis ogólny
Kit is used to anaylze β glucuronidase (E.coli GUS gene) expression in transformed plants.
Zastosowanie
The E. coli GUS gene is extensively used to analyze gene expression in transformed plants. Plants have low intrinsic GUS activity, and the E. coli gene is quite stable in plant cells. As a tag, GUS remains active at the N-terminus of fusion proteins.
Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.
Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.
Cechy i korzyści
- Histochemical staining of plant tissues expressing the E. coli GUS enzyme
- Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability
- GUS does not interfere with plant cell function or viability
Inne uwagi
The substrate used in this kit is X-GlcA (5-bromo-4-chloro-3-indolyl β-D-glucuronide) which results in an insoluble indigo-blue precipitate in transfected cells and tissues.
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Tylko elementy zestawu
Numer produktu
Opis
- 2× Fixation Buffer 25 mL
- 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98% 1 mL/vial
- Reagent A for gus staining kit 50 mL
- Reagent B for gus staining kit 200 μL
- Reagent C for gus staining kit 200 μL
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Aquatic Chronic 3
Kod klasy składowania
11 - Combustible Solids
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The GUS reporter gene system is already a powerful tool for the assessment of gene activity in transgenic plants. Further developments may lead to routine in vivo analysis and fusion genetics.
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