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APOAF

Sigma-Aldrich

Annexin V-FITC Apoptosis Detection Kit

Synonim(y):

Annexin V-FITC, Apoptosis probe FITC

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About This Item

Kod UNSPSC:
12352207
NACRES:
NA.84

zastosowanie

(20 tests)

Poziom jakości

opakowanie

pkg of 1 kit

metody

flow cytometry: suitable

Zastosowanie

cell analysis
detection

metoda wykrywania

fluorometric

Warunki transportu

wet ice

temp. przechowywania

2-8°C

Opis ogólny

Annexin V-FITC kit allows fluorescent detection of annexin V bound to apoptotic cells and quantitative determination by flow cytometry. The AnnexinV-FITC kit uses annexin V conjugated with fluorescein isothiocyante (FITC) to label phosphatidylserine sites on the membrane surface. The kit includes propidium iodide (PI) to label the cellular DNA in necrotic cells where the cell membrane has been totally compromised. This combination allows the differentiation among early apoptotic cells (annexin V positive, PI negative), necrotic cells (annexin V positive, PI positive), and viable cells (annexin V negative, PI negative).

Zastosowanie

Annexin V-FITC Apoptosis Detection Kit was used:
  • in staining of LNCaP prostate cancer cells for measuring the G. lucidum extracts activity during the treatment of prostate cancer.
  • for tumor cell labelling to study the inhibitory activity of DBP-maf (Vitamin D binding protein-macrophage activating factor) on prostate tumor cells.
  • for indirect measurement of flippase activity.

Cechy i korzyści

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL.
  • Rapid labeling of cells. Cell staining takes only 10 minutes.
  • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
  • Propidium iodide secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Inne uwagi

Allow all components to reach room temperature before use.
This page may contain text that has been machine translated.

Tylko elementy zestawu

Numer produktu
Opis

  • APOAFA

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Numer produktu
Opis
Cennik

Kod klasy składowania

10 - Combustible liquids


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Produkty

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Testy apoptozy komórkowej do wykrywania zaprogramowanej śmierci komórki przy użyciu aneksyny V, kaspazy i testów fragmentacji DNA TUNEL.

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