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C4478
S-Gal®/LB Agar Blend
reagent for selection of recombinant bacterial clones
Synonim(y):
Agar Blend
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About This Item
Kod UNSPSC:
41106200
NACRES:
NA.85
Polecane produkty
klasa czystości
for molecular biology
Poziom jakości
sterylność
non-sterile
Postać
powder
metody
microbiological culture: suitable
przydatność
suitable for β-galactosidase test
nonselective for Escherichia coli
nonselective for coliforms
Zastosowanie
food and beverages
microbiology
temp. przechowywania
room temp
Powiązane kategorie
Opis ogólny
S-Gal® (sodium salt) is an autoclavable, water-soluble, chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. S-gal® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Zastosowanie
S-Gal®/LB Agar Blend has been used for the identification of recombinant bacterial colonies.
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. S-Gal® (sodium salt) is water-soluble, autoclavable and can be added to bacterial broth containing agar prior to autoclaving.
Cechy i korzyści
- More intense color contrast than X-gal
- Water-soluble and autoclavable for easiest use
- Convenient, pre-mixed media
Komponenty
Ingredients (g/L)
Tryptone, 10
Yeast extract, 5
Sodium chloride, 10
Agar, 12
S-Gal, 0.3
Ferric ammonium citrate, 0.5
IPTG, 0.03
Tryptone, 10
Yeast extract, 5
Sodium chloride, 10
Agar, 12
S-Gal, 0.3
Ferric ammonium citrate, 0.5
IPTG, 0.03
Zasada
When S-Gal® is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal® and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies.
Uwaga dotycząca przygotowania
Suspend contents of one packet in 500 ml distilled or deionized water. Sterilize by autoclaving for 15 to 20 minutes at 121-124°C. For microwaving, heat suspended mix until initial boiling. Mix well. Heat for short intervals with mixing until agar component is in solution. Do not allow boiling for extended periods of time. Antibiotics should be added following autoclaving or microwaving, after cooling to 48-52°C.
Inne uwagi
The ferric or Fe3+ ion is required for color development and must be added to any S-Gal®
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
Informacje prawne
S-GAL is a registered trademark of Merck KGaA, Darmstadt, Germany
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produkt powiązany
Numer produktu
Opis
Cennik
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
Certyfikaty analizy (CoA)
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