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A7646
Apyrase from potatoes
ATPase ≥60 units/mg protein, lyophilized powder (partially purified)
Synonim(y):
Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase
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About This Item
Polecane produkty
pochodzenie biologiczne
potato
typ
Grade III
Formularz
lyophilized powder (partially purified)
ATPase activity
≥60 units/mg protein
masa cząsteczkowa
~49 kDa by gel filtration
skład
Protein, 30-60%
rozpuszczalność
H2O: soluble 10 mg/mL, slightly hazy
Zastosowanie
diagnostic assay manufacturing
obecność zanieczyszczeń
Acid Phosphatase ≤5% of base activity
temp. przechowywania
−20°C
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Zastosowanie
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation. The enzyme has been used to assess the possibility of ATP involvement in intestinal lamina propria Th17 cell differentiation.
At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.
Reaction: ATP → ADP+Pi → AMP+2Pi.
Działania biochem./fizjol.
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa.
Opakowanie
Sold on the basis of ATPase units
Inne uwagi
Mixture of high and low ratio isoenzymes.
Jakość
Contains ≤ 5% acid phosphatase.
Definicja jednostki
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
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Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Resp. Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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