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MAK113

Sigma-Aldrich

ATPase/GTPase Activity Assay Kit

sufficient for 200 colorimetric tests

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About This Item

Kod UNSPSC:
12161503
NACRES:
NA.84

zastosowanie

sufficient for 200 colorimetric tests

metoda wykrywania

colorimetric

powiązane choroby

cancer

temp. przechowywania

2-8°C

Opis ogólny

ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate. These enzymes play key roles in transport, signal transduction, protein biosynthesis, and cell differentiation.

Cechy i korzyści

Compatible with high-throughput handling systems.

Przydatność

Suitable for the detection of ATPase and GTPase activity and for the screening of ATPase and GTPase inhibitors.

Zasada

The ATPase/GTPase Activity Assay kit provides a simple and direct procedure for measuring ATPase/GTPase activity in a microplate format. This kit uses a single reagent formulation to accurately determine enzyme activity in 30 minutes at room temperature. The malachite green reagent forms a stable dark green color with free phosphate liberated by the enzymes resulting in a colorimetric product, measured at 620 nm (600-660 nm), proportional to the enzyme activity present One unit of activity is the amount of enzyme that catalyzes the production of 1 μmole of free phosphate per minute under the assay conditions.
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Piktogramy

Corrosion

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Met. Corr. 1

Kod klasy składowania

8B - Non-combustible corrosive hazardous materials

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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