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Merck

A3782

Sigma-Aldrich

Albumin from human serum

lyophilized powder, Fatty acid free, Globulin free, ≥99% (agarose gel electrophoresis)

Synonim(y):

HSA

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About This Item

Numer CAS:
Numer WE:
Numer MDL:
Kod UNSPSC:
12352202
eCl@ss:
42010201
NACRES:
NA.25

pochodzenie biologiczne

human

Poziom jakości

Próba

≥99% (agarose gel electrophoresis)

Postać

lyophilized powder

masa cząsteczkowa

monomer calculated mol wt 66478 Da

metody

ELISA: suitable
tissue culture: suitable
western blot: suitable

zanieczyszczenia

HIV I and HIVII, HCV and HBsAg, tested negative
≤0.1% Fatty acids

rozpuszczalność

H2O: soluble 50 mg/mL

numer dostępu UniProt

temp. przechowywania

2-8°C

InChI

1S/C3F8/c4-1(5,2(6,7)8)3(9,10)11

Klucz InChI

QYSGYZVSCZSLHT-UHFFFAOYSA-N

informacje o genach

human ... ALB(213)

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Opis ogólny

Albumin is the most abundant protein in blood plasma, produced in the liver.

Albumin is a known carrier of fatty acids (FA). Thus control over specific FA′s for cell culture is important, as different cell lines can differ in their sensitivity to particular fatty acids. Fatty acid-free human serum albumin (HSA) is therefore useful for cell culture studies where specific fatty acid content must be strictly controlled, so that researchers can use particular fatty acids specific to their cell lines. Fatty acid-free albumin also allows for optimal and maximum binding sites for using specific fatty acids in cell culture. The use of FA-free HSA also addresses concerns about endogenous FA′s potentially in non-FA-free HSA.
Albumin is the most copious plasma protein in humans. Albumin turnover is seen in infants with iron deficiency anemia. Serum albumin is a reliable prognostic indicator in liver disease. Oxidative damage of albumin is associated with advanced liver disease.

Albumin is widely used as a blocking agent in immunoassays and immunodetection procedures, and as a carrier protein for dilution of antibodies. Awareness of potential trace endogenous content of globulins / IgG in human serum albumin (HSA) is therefore important, because such trace globulins / IgG may serve as antigens for secondary antibodies in immunodetection work. This product undergoes testing for low globulin content / status as essentially globulin-free.

Zastosowanie

Albumin from human serum has been used in the preparation of glycated human serum albumin (gHSA) in a glucose solution. It has also been used in the study to estimate the impact of surface nanotopography and chemical composition on blood compatibility.
Albumin was used to test its effect on the in vitro bactericidal activity of cefditoren against penicillin-resistant Streptococcus pneumonia. It has been clinically used in serious and often life-threatening conditions, such as shock and blood loss due to trauma, burns, and surgery. It was used also to test the effect of non-enzymatic glycation on the unfolding of human serum albumin.

Działania biochem./fizjol.

Serum albumin functions as a carrier protein for steroids, fatty acids, and thyroid hormones, and is vital in regulating the colloidal osmotic pressures of blood. Albumin is also seen to bind to exogenous substances, particularly drugs (e.g., ibuprofen, warfarin), and strongly influence their pharmacokinetics. Oxidative stress leading to changes in the redox state of albumin has widely varied effects on its physiological function.

Cechy i korzyści

  • Fatty acid-free / low fatty acid content
  • Globulin-free / low globulin content

Inne uwagi

View more information on human serum albumin.

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
This page may contain text that has been machine translated.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Claudio Dalvit et al.
Magnetic resonance in chemistry : MRC, 55(2), 106-114 (2016-08-16)
Ligand-based
Fibrillation and Polymorphism of Human Serum Albumin
Bio-nanoimaging, 77(2), 345-362 (2014)
Competitive adsorption of fibrinogen and albumin and blood platelet adhesion on surfaces modified with nanoparticles and/or PEO
Nonckreman C J, et al.
Colloids and Surfaces. B, Biointerfaces, 77(2), 139-149 (2010)
Isabel Correia et al.
Chemistry, an Asian journal, 12(16), 2062-2084 (2017-06-27)
[VO(acac)
Deanna L Mendez et al.
Archives of biochemistry and biophysics, 444(2), 92-99 (2005-11-29)
We monitored the unfolding of human serum albumin (HSA) and glycated human serum albumin (gHSA) subjected to guanidine hydrochloride (GndHCl) by using fluorescence and circular dichroism (CD) spectroscopy. A two-state model with sloping baselines best described the Trp-214 fluorescence unfolding

Produkty

This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

Lipid Induced Insulin Resistance

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