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D9156

Sigma-Aldrich

Deoxyribonucleic acid, single stranded from salmon testes

For hybridization

Synonim(y):

jednoniciowa matryca DNA

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About This Item

Numer CAS:
68938-01-2
Numer MDL:
MFCD00130921
Kod UNSPSC:
41106310
eCl@ss:
32160414
NACRES:
NA.52

pochodzenie biologiczne

fish testis (salmon)

klasa czystości

for molecular biology

Próba

9-11 mg/mL (DNA concentration)

Formularz

solution

stężenie

10 mg/mL in H2O

Warunki transportu

dry ice

temp. przechowywania

−20°C

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Opis ogólny

A ready-to-use solution of high quality single-stranded template DNA isolated from the testes of salmon. The solution is supplied at a concentration of 9 - 12 mg/ml .

Zastosowanie

Deoxyribonucleic acid, single stranded from salmon testes is suitable for use as a blocking agent in Southern hybridizations. It was used for in situ hybridization histochemistry of bone marrow biopsy samples and human blood monocyte subpopulations.
For use as a blocking agent in Northern and Southern blotting.
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.

In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.

Uwaga dotycząca przygotowania

This DNA is ethanol precipitated and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.

Inne uwagi

DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
SLCL2755
SLCG2425
SLCB8121
SLBX8964
SLBZ0079

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Jeśli potrzebujesz konkretnej wersji, możesz wyszukać konkretny certyfikat według numeru partii lub serii.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

H W Ziegler-Heitbrock et al.
Blood, 79(2), 503-511 (1992-01-15)
Cytokine expression was analyzed in CD14++ regular monocytes and in the novel subset of CD14+/CD16+ small monocytes. Biologic activity for tumor necrosis factor (TNF), interleukin-1 (IL-1), and IL-6 in the supernatant of elutriator-enriched, cell sorter-purified small monocytes was about 10-fold
J W Said et al.
Blood, 90(11), 4278-4282 (1997-12-31)
We have recently demonstrated the presence of Kaposi's sarcoma-associated herpesvirus (KSHV) in cultured bone marrow (BM) stromal dendritic cells from all patients with myeloma studied. To show that these findings were not an artifact of tissue culture, we performed in
N Milne et al.
FEMS yeast research, 20(2) (2019-09-27)
We present a teaching protocol suitable for demonstrating the use of EasyClone and CRISPR/Cas9 for metabolic engineering of industrially relevant yeasts Saccharomyces cerevisiae and Yarrowia lipolytica, using β-carotene production as a case study. The protocol details all steps required to
Emilia Huerta-Sánchez et al.
Nature, 512(7513), 194-197 (2014-07-22)
As modern humans migrated out of Africa, they encountered many new environmental conditions, including greater temperature extremes, different pathogens and higher altitudes. These diverse environments are likely to have acted as agents of natural selection and to have led to
Maarten Holkers et al.
Nature methods, 11(10), 1051-1057 (2014-08-26)
Engineered sequence-specific nucleases and donor DNA templates can be customized to edit mammalian genomes via the homologous recombination (HR) pathway. Here we report that the nature of the donor DNA greatly affects the specificity and accuracy of the editing process
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