About This Item
Kod UNSPSC:
41106514
Polecane produkty
pochodzenie biologiczne
human lymph
Poziom jakości
Formularz
liquid
tryb wzrostu
Adherent
kariotyp
Not specified
morfologia
Epithelial
produkty
Not specified
receptory
Not specified
metody
cell culture | mammalian: suitable
powiązane choroby
cancer
Warunki transportu
dry ice
temp. przechowywania
−196°C
Opis ogólny
COLO 699 N cells, a non-small cell lung cancer cell line is useful in subcutaneous xenografts studies to mimic in vivo model of human lung cancer. It finds applications in stable isotope-based proteome analysis.
Pochodzenie linii komórkowej
Human lung cancer
Opis linii komórkowej
Derived from the pleural fluid of a 57 year old female with lung cancer. Please note: This cell line was found to be indistinguishable from cell lines RPMI 7932 & COLO 738 by STR PCR DNA profiling. At ECACC the STR PCR profile of all stocks of this cell line match the profile of the original source material received at ECACC. However, it is not clear if the cell lines COLO 699N, RPMI 7932 & COLO 738 were originally derived from the same individual as the profiles suggest. The cell lines COLO 699N, RPMI 7932 & COLO 738 were received from the same depositor. If we are able to obtain clarification of the relationship between these cell lines this information will be updated.
Zastosowanie
COLO 699 N has been used in cytotoxicity studies with Tegaran and 13-methyltetradecanoic acid (13-MTD).
Tumourigenicity studies
Profil DNA
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 12
D5S818: 11,12
D7S820: 11,13
THO1: 9,9.3
TPOX: 8
vWA: 17
CSF1PO: 10
D13S317: 11
D16S539: 12
D5S818: 11,12
D7S820: 11,13
THO1: 9,9.3
TPOX: 8
vWA: 17
pożywka hodowlana
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Rutyna subkultury
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Inne uwagi
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Oświadczenie o zrzeczeniu się odpowiedzialności
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Certyfikaty analizy (CoA)
Lot/Batch Number
Przepraszamy, ale COA dla tego produktu nie jest aktualnie dostępny online.
Proszę o kontakt, jeśli potrzebna jest pomoc Obsługa Klienta
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Kathrin Grundner-Culemann et al.
Journal of proteomics, 130, 1-10 (2015-09-13)
Non-small cell lung cancer (NSCLC) cell lines are widely used model systems to study molecular aspects of lung cancer. Comparative and in-depth proteome expression data across many NSCLC cell lines has not been generated yet, but would be of utility
H G Morse et al.
Cancer genetics and cytogenetics, 69(2), 108-112 (1993-09-01)
Melanoma cell lines initiated from metastases excised at the same time from multiple sites in a patient reflect a single clone origin. The similarities of the karyotypes of these lines depend upon the site of excision, the selective survival in
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