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Key Documents

94072246

RPMI 7932 cell line

94072246, human lung effusion, Epithelial-like morphology

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
41106514

product name

RPMI 7932 cell line,

pochodzenie biologiczne

human lung (effusion)

Postać

liquid

tryb wzrostu

Adherent

kariotyp

Not specified

morfologia

Epithelial-like

produkty

Plasminogen activator

receptory

Not specified

metody

cell culture | mammalian: suitable

powiązane choroby

cancer

Warunki transportu

dry ice

temp. przechowywania

−196°C

Pochodzenie linii komórkowej

Human pleural effusion from a patient with malignant melanoma

Opis linii komórkowej

Derived from the pleural effusion of a 36 year old female with malignant melanoma. Cell line produces plasminogen activator. This cell line was found to be indistinguishable from cell lines COLO 699N & COLO 738 by STR PCR DNA profiling. At ECACC the STR PCR profile of all stocks of this cell line match the profile of the original source material received at ECACC. However, it is not clear if the cell lines COLO 699N, RPMI 7932 & COLO 738 were originally derived from the same individual as the profiles suggest. The cell lines COLO 699N, RPMI 7932 & COLO 738 were received from the same depositor. If we are able to obtain clarification of the relationship between these cell lines this information will be updated.

Zastosowanie

Tumourigenicity studies

pożywka hodowlana

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-6x10,000 cells/cm2using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Inne uwagi

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

B K Bhuyan et al.
Cancer research, 46(4 Pt 1), 1688-1693 (1986-04-01)
Our interest in prostaglandins (PGs) as antitumor agents stemmed from the report of Bregman and Meyskens (Cancer Res., 43: 1642-1645, 1983) that PGA1, PGA2, and PGD2 inhibited colony formation by human melanoma cells obtained from fresh biopsies of melanoma patients.
B Sharma
Cancer research, 37(12), 4660-4668 (1977-12-01)
Tumor cell fractions isolated from tumor lines SH-3 (breast carcinoma) and RPMI-7932 (malignant melanoma) by differential centrifugations were capable of transforming lymphocytes into cytotoxic effector cells. Lymphocytes cultured alone in human AB plasma did not become cytotoxic to tumor cells.
Generation of cytotoxic lymphocytes against human tumor cells in vitro by various soluble microbial extracts.
B Sharma et al.
Journal of the National Cancer Institute, 63(2), 341-346 (1979-08-01)
C W Porter et al.
Cancer research, 54(22), 5917-5924 (1994-11-15)
Certain N-alkylated analogues of the natural polyamine spermine, such as N1,N11-diethylnorspermine (DENSPM), rapidly deplete intracellular polyamine pools by down-regulating the biosynthetic enzymes, ornithine decarboxylase and S-adenosylmethionine decarboxylase, and by potently up-regulating the polyamine catabolizing enzyme, spermidine/spermine N1-acetyltransferase. On the basis
J A Eisman et al.
Cancer research, 47(1), 21-25 (1987-01-01)
It has been demonstrated previously that several human cancer cell lines possess specific, high affinity receptors for 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3, calcitriol] and that 1,25-(OH)2D3 and certain of its metabolites inhibit the growth in vitro of several human breast cancer and

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